T&R BIOFAB

Organoids and Mini Tissues

3D-bioprinted skin

T&R
Human skin

Research & Development

Our 3D-bioprinted skin model is composed of epidermis and dermis similar to human skin, with expression of markers found in real skin. In addition, the dermis is made of porcine skin-derived decellularized extracellular matrix, one of our core technologies, thus exhibiting higher similarity to real skin than the existing collagen-based skin models.

Scale Bar : 100 µm

Uses

01Model for evaluating the toxicity and efficacy of cosmetics and the toxicity of chemicals

02Model for evaluating efficacy such as wound healing ability of medicines or medical devices

03Artificial skin graft for the treatment of burns and chronic wounds

1. Jang, K. S., Park, S. J., Choi, J. J., Kim, H. N., Shim, K. M., Kim, M. J., ... & Moon, S. H. (2021). Therapeutic efficacy of artificial skin produced by 3d bioprinting. Materials, 14(18), 5177.
2. Won, J. Y., Lee, M. H., Kim, M. J., Min, K. H., Ahn, G., Han, J. S., ... & Shim, J. H. (2019). A potential dermal substitute using decellularized dermis extracellular matrix derived bio-ink. Artificial Cells, Nanomedicine, and Biotechnology, 47(1), 644-649.
3. Girardeau-Hubert, S., Lynch, B., Zuttion, F., Label, R., Rayee, C., Brizion, S., ... & Soeur, J. (2022). Impact of microstructure on cell behavior and tissue mechanics in collagen and dermal decellularized extra-cellular matrices. Acta Biomaterialia, 143, 100-114.

Liver organoid

Vascular endothelial cellsHepatic cells
Hepatic cellsVascular endothelial cells

Research & Development

Using our patented 3D bioprinting preset extrusion method, we are manufacturing phenotypically-relevant analogs of human liver lobules. Our 3D liver organoids are hepatocytes and vascular cells arranged with a precision that is difficult to achieve with conventional 3D bioprinting technology, and its shape and function are maintained for a longer period of time compared to existing liver tissue analogues.

Left - Scale bar : 400 µm
Right - Scale bar : 100 µm

Uses

01Evaluation of hepatotoxicity of drugs in new drug development

02Development of disease models such as NASH

03Treatment of damaged liver tissues through the liver cell therapy

1. Kang, D., Ahn, G., Kim, D., Kang, H. W., Yun, S., Yun, W. S., ... & Jin, S. (2018). Pre-set extrusion bioprinting for multiscale heterogeneous tissue structure fabrication. Biofabrication, 10(3), 035008.
2. Kang, D., Hong, G., An, S., Jang, I., Yun, W. S., Shim, J. H., & Jin, S. (2020). Bioprinting of multiscaled hepatic lobules within a highly vascularized construct. Small, 16(13), 1905505.
3. Hong, G., Kim, J., Oh, H., Yun, S., Kim, C. M., Jeong, Y. M., ... & Jin, S. (2021). Production of Multiple Cell‐Laden Microtissue Spheroids with a Biomimetic Hepatic‐Lobule‐Like Structure. Advanced Materials, 33(36), 2102624.